Literature summary for 2.7.7.B22 extracted from

Ni, J.; Wangensteen, K.; Nelsen, D.; Balciunas, D.; Skuster, K.; Urban, M.; Ekker, S.
Active recombinant Tol2 transposase for gene transfer and gene discovery applications (2016), Mob. DNA, 7, 006 .

Search for more literature for 2.7.7.B22

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HeLa cell	Oryzias latipes

Organism

Organism	UniProt	Comment	Textmining
Oryzias latipes	Q9PTV0 and Q9PTV1	Q9PTV0 i.e. shorter mRNA Tol2-S encoded by exons 2-4, Q9PTV1 i.e. longer mRNA Tol2-L encoded by exons 1-4. Tol2-M mRNA encodes a predicted protein that is shorter at the N-terminus than Tol2-L	-

Synonyms

Synonyms	Comment	Organism
Tol2	-	Oryzias latipes
Tpase	-	Oryzias latipes

General Information

General Information	Comment	Organism
physiological function	following microinjection using a zebrafish embryo test system, purified Tol2-M transposase protein readily catalyzes gene transfer in both somatic and germline tissues in vivo. Purified Tol2-M transposase can promote both in vitro cutting and pasting in a defined system lacking other cellular factors. A primary sequence of the target site is likely to play a critical role in Tol2 integration site selection	Oryzias latipes

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Release 2023.1 (January 2023)